生化学研究室

Analysis of thermostability for seven Phe to Ala and six Pro to Gly mutants in the Fab constant region of adalimumab.
M. Yoshikawa, H. Nakamura, N. Oda-Ueda, T. Ohkuri
J. Biochem. 174 (4), 345-353 (2023)

Effect of an intermolecular disulfide bond introduced into the first loop of CH1 domain of Adalimumab Fab on thermal stability and antigen-binding activity.
M. Yoshikawa, H. Nakamura, N. Oda-Ueda, T. Ueda, T. Ohkuri
J. Biochem. 172 (1), 49-56 (2022)

A comprehensive analysis of novel disulfide bond introduction site into the constant domain of human Fab.
H. Nakamura, M. Yoshikawa, N. Oda-Ueda, T. Ueda, T. Ohkuri
Sci. Rep. 11 (1), 12937 (2021)
Glycosylation decreases aggregation and immunogenicity of adalimumab Fab secreted from Pichia pastoris.
H. Nakamura, M. Kiyoshi, M. Anraku, N. Hashii, N. Oda-Ueda, T. Ueda, T. Ohkuri
J. Biochem. 169 (4), 435-443 (2021)

C-Terminal Cysteine PEGylation of Adalimumab Fab with an Engineered Interchain SS Bond.
H. Nakamura, M. Anraku, N. Oda-Ueda, T. Ueda, T. Ohkuri
Biol. Pharm. Bull. 43 (3), 418-423 (2020)
Discovery of the gene encoding a novel small serum protein (SSP) of Protobothrops flavoviridis and the evolution of SSPs.
K. Inamaru, A. Takeuchi, M. Maeda, H. Shibata, Y. Fukumaki, N. Oda-Ueda, S. Hattori, M. Ohno, T. Chijiwa
Toxins (Basal) 12 (3), 177 (2020)
Crystal structure of the complex of the interaction domains of Escherichia coli DnaB helicase and DnaC helicase loader: structural basis implying a distortion-accumulation mechanism for the DnaB ring opening caused by DnaC binding.
K. Nagata, A. Okada, J. Ohtsuka, T. Ohkuri, Y. Akama, Y. Sakiyama, E. Miyazaki, S. Horita, T. Katayama, T. Ueda, and M. Tanokura
J. Biochem. 167 (1), 1-14 (2020)

Alternative mRNA splicing in three venom families underlying a possible production of divergent venom proteins of the Habu snake, Protobothrops flavoviridis.
T. Ogawa, N. Oda-Ueda, K. Hisata, H. Nakamura, T. Chijiwa, S. Hattori, A. Isomoto, H. Yugeta, S. Yamasaki, Y. Fukumaki, M. Ohno, N. Satoh, H. Shibata
Toxins (Basal) 11 (10), pii. E581 (2019)
Unique structure (construction and configuration) and evolution of the array of small serum protein genes of Protobothrops flavoviridis snake.
T. Chijiwa, K. Inamaru, A. Takeuchi, M. Maede, K. Yamaguchi, H. Shibata, S. Hattori, N. Oda-Ueda, M. Ohno
Biosci. Rep. 39 (7), pii:BSR20190560 (2019)
SDS-induced oligomerization of Lys49-phospholipase A₂ from snake venom.
T. Matsui, S. Kamata, K. Ishii, T. Maruno, N. Ghanem, S. Uchiyama, K. Kato, A. Suzuki, N. Oda-Ueda, T. Ogawa, Y. Tanaka
Sci. Rep. 9 (1), 2330 (2019)
A method to induce hen egg lysozyme-specific humoral immune tolerance in mice by pre-exposition with the protein's oligomers.
T. Ohkuri, N. Yuge, K. Sato, T. Ueda
Biochem. Biophys. Rep. 20, 100679 (2019)

The habu genome reveals accelerated evolution of venom protein genes.
H. Shibata, T. Chijiwa, N. Oda-Ueda, H. Nakamura, K. Yamaguchi, S. Hattori, K. Matsubara, Y. Matsuda, A. Yamashita, A. Isomoto, K. Mori, K. Tashiro, S. Kuhara, S. Yamasaki, M. Fujie, H. Goto, R. Koyanagi, T. Takeuchi, Y. Fukumaki, M. Ohno, E. Shoguchi, K. Hisata, N. Satoh, T. Ogawa
Sci. Rep. 8 (1), 11300 (2018)
Introduction of a glycosylation site in the constant region decreases the aggregation of adalimumab Fab.
H. Nakamura, N. Oda-Ueda, T. Ueda, T. Ohkuri
Biochem. Biophys. Res. Commun. 503 (2), 752-756 (2018)
A novel engineered interchain disulfide bond in the constant region enhances the thermostability of adalimumab Fab.
H. Nakamura, N. Oda-Ueda, T. Ueda, T. Ohkuri
Biochem. Biophys. Res. Commun. 495 (1), 7-11 (2018)

Relationship between the magnitude of IgE production in mice and conformational stability of the house dust mite allergen, Der p 2.
H. Nakamura, T. Ohkuri, T. So, T. Ueda
Biochim. Biophys. Acta. 1860 (10), 2279-2284 (2016)

Interisland variegation of venom [Lys⁴⁹]phospholipase A₂ isozyme genes in Protobothrops genus snakes in the southwestern islands of Japan.
K. Yamaguchi, T. Chijiwa, T. Yamamura, N. Ikeda, T. Yatsui, S. Hayama, S. Hattori, N. Oda-Ueda, M. Ohno
Toxicon 107 (Pt B), 210-216 (2015)
Role of the osmolyte taurine on the folding of a model protein, hen egg white lysozyme, under a crowding condition.
Y. Abe, T. Ohkuri, S. Yoshitomi, S. Murakami, T. Ueda.
Amino Acids 47 (5), 909-915 (2015)

The finding of a group IIE phospholipase A₂ gene in a specified segment of Protobothrops flavoviridis genome and its possible evolutionary relationship to group IIA phospholipase A₂ genes.
K. Yamaguchi, T. Chijiwa, N. Ikeda, H. Shibata, Y. Fukumaki, N. Oda-Ueda, S. Hattori, M. Ohno
Toxins (Basel) 6 (12), 3471-3487 (2014)
Epithelium specific ETS transcription factor, ESE-3, of Protobothrops flavoviridis snake venom gland transactivates the promoters of venom phospholipase A₂ isozyme genes.
H. Nakamura, T. Murakami, S. Hattori, Y. Sakaki, T. Ohkuri, T. Chijiwa, M. Ohno, N. Oda-Ueda
Toxicon 92, 133-139 (2014)
Discovery of a novel vascular endothelial growth factor (VEGF) with no affinity to heparin in Gloydius tsushimaensis venom.
H. Nakamura, T. Murakami, T. Imamura, M Toriba, T. Chijiwa, M. Ohno, N. Oda-Ueda
Toxicon 86, 107-115 (2014)

Suppression of severe lesions, myonecrosis and hemorrhage, caused by Protobothrops flavoviridis venom with its serum proteins.
T. Chijiwa, S. So, S. Hattori, A. Yoshida, N. Oda-Ueda, M. Ohno
Toxicon 76, 197-205 (2013)
Characterization of deamidation at Asn138 in L-chain of recombinant humanized Fab expressed from Pichia pastoris.
T. Ohkuri, E. Murase, S.L. Sun, J. Sugitani, T. Ueda
J. Biochem. 154 (4), 333-340 (2013)
Domain separation and characterization of PriC, a replication restart primosome factor in Escherichia coli.
T. Aramaki, Y. Abe, T. Ohkuri, T. Mishima, S. Yamashita, T. Katayama, T. Ueda
Genes Cells. 18 (9), 723-732 (2013)
Mechanism for retardation of amyloid fibril formation by sugars in Vλ6 protein.
M. Abe, Y. Abe, T. Ohkuri, T. Mishima, A. Monji, S. Kanba, T. Ueda
Protein Sci. 22 (4), 467-474 (2013)
Structual characteristics and evolution of the Protobothrops elegans pancreatic phospholipase A₂ gene in contrast with those of Protobothrops genus venom phospholipase A₂ genes.
T. Chijiwa, H. Nakasone, S. Irie, N. Ikeda, K. Tomoda, N. Oda-Ueda, S. Hattori, M. Ohno
Biosci. Biotechnol. Biochem. 77 (1), 97-102 (2013)
Preparation and characterization of a monoclonal antibody against the refolded and functional extracellular domain of rat P2X4 receptor.
T. Igawa, S. Higashi, Y. Abe, T. Ohkuri, H. Tanaka, S. Morimoto, T. Yamashita, M. Tsuda, K. Inoue, T. Ueda
J. Biochem. 153 (3), 275-282 (2013)

Comparative analysis of cells and proteins of pumpkin plants for the control of fruit size.
Y. Ohshima, Y. Nakata, G. Taniguchi, S. Takazak, N. Oda-Ueda, K. Miyahara
J. Biosci. Bioeng. 114 (3), 334-341 (2012)

Aggregates with lysozyme and ovalbumin show features of amyloid-like fibrils.
Y. Sugimoto, Y. Kamada, Y. Tokunaga, H. Shinohara, M. Matsumoto, T. Kusakabe, T. Ohkuri, T. Ueda
Biochem. Cell Biol. 89 (6), 533-544 (2011)
Identification and evolution of venom phospholipaseA₂ inhibitors from Protobothrops elegans serum.
S. So, T. Murakami, N. Ikeda, T. Chijiwa, N. Oda-Ueda, T. Kuraishi, S. Hattori, M. Ohno
Biosci. Biotech. Biochem. 75 (3), 480-488 (2011)
A [Lys⁴⁹]phospholipaseA₂ from Protobothrops flavoviridis venom induces caspase-independent apoptotic cell death accompanied by rapid plasma membrane rupture in human leukemia cells.
T. Murakami, N. Kamikado, R. Fujimoto, K. Hamaguchi, H. Nakamura, T. Chijiwa, M. Ohno, N. Oda-Ueda.
Biosci. Biotech. Biochem. 75 (5), 864-870 (2011)