regulated by PTH (1-34). In Caco-2 cells, PTH (1-34) down-regulated the expression of CYP3A mRNA, but an inactive PTH derivative (13-34) had no effect. 8-Bromo-cyclic adenosine monophosphate, a membrane-permeable cAMP analog, reduced mRNA expression of CYP3A whereas the inhibitors of PI3K, NF-κB, PKC and PKA reversed the PTH-induced CYP3A down-regulation. These results suggest that PTH down-regulates CYP3A through multiple signaling pathways, including the PI3K/PKC/PKA/NF-κB pathway after the elevation of intracellular cAMP, and the effect of PTH can be prevented by cinacalcet treatment. () Quantitative determination of polysulfide in albumins, plasma proteins and biological fluid samples using a novel combined assays approach. Ikeda M () Ishima Y () Shibata A () Chuang VTG () Sawa T () Ihara H () Watanabe H () Xian M () Ouchi Y () Shimizu T () Ando H () Ukawa M () Ishida T () Akaike T () Otagiri M () Maruyama T () Anal Chim Acta., Vol.969, pp.18-25 2017 (29) doi: 10.1016/j.aca.2017.03.027 Hydrogen sulfide (H2S) signaling involves polysulfide (RSSnSR') formation on various proteins. However, the current lack of sensitive polysulfide detection assays poses methodological challenges for understanding sulfane sulfur homeostasis and signaling. We developed a novel combined assay by modifying Sulfide Antioxidant
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